Fig. 7. TUDC blocks the GCDC-induced formation of reactive oxygen species. Primary rat hepatocytes were cultured for 24 h, loaded with DCF as described in materials and methods and exposed to GCDC (50 µmol/l) for 15 min. Where indicated, hepatocytes were pre-incubated with TUDC (50 µmol/l) for 30 min before hepatocytes were exposed to GCDC in the presence of TUDC. Fluorescence was collected by epifluorescence microscopy and fluorescence intensities were quantified using AxioVision Software. ROS levels in GCDC or TUDC-exposed hepatocytes are given relative to the control. Data are mean values ± SEM of n≥3 independent experiments. * statistically significantly different compared to the untreated control. n.s.: not statistically significantly different.